Detection of Culturable Bacteria in Greenhouse Grown Romaine Lettuce Using the Light Scattering Technology (BEAM)- Hansel Mina and Amanda Deering

Technologies for rapid detection and identification of bacterial communities, especially for pathogens, are crucial for securing a safe food supply. The light-scattering technology (BEAM) incorporates the traditional culture-based approach as a part of the detection procedure and is an easier and faster method for detection and identification of bacteria based on the morphological characteristics of the colonies.  A light scatter pattern library was created based on the most common bacterial genera associated with different varieties of romaine lettuce with each scatter patterns being unique to each bacteria genus. However, scatter patterns obtained from the same bacterial colony was found to change depending on the incubation time due to changes in colony size and morphological characteristics. Therefore, the scatter patterns for the different bacterial genera were evaluated at 12, 21, 32 and 72 hours of incubation for fast, intermediate, medium and slow growth rates to create a library to be able to detect various bacteria using the BEAM technology.

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